| 1. | Conclusion the enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes
结论：酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。 |
| 2. | After identification with both pcr and enzyme digestion methods , accurate recombinants were sent to detect their sequences
将经过pcr和双酶切鉴定的重组克隆进行序列测定。 |
| 3. | After repeated adherence and enzyme digestion , the cells attained the requirement of morphological purification with a purity of more than 95 %
经台盼蓝拒染计数，将细胞按1 10 ^ 6接种于培养瓶，传代培养。 |
| 4. | It was confirmed by restriction enzyme digestion analysis that egfp fusion expression plasmids of scfvs were successfully constructed
的序列正确，经酶切鉴定证实成功地构建了绿色荧光蛋白基因融合表达载体。 |
| 5. | E2 gene was cut with restriction endonuclease from the recombination plasmid t - e2 , and subcloned into the expression vector ppic9 ' s mcs , which was identified by enzyme digestion and pcr amplification
将重组质粒t - e2的e2基因酶切后，亚克隆到表达载体ppic9上，经酶切和pcr鉴定，命名为ppic9 - e2 。 |
| 6. | The expression vector ppiczaa with the poifn - a gene was constructed by restrict enzyme digestion and ligation , then transformed into e . coli jm109 in order to extract recombinant plasmid from jm109
将此基因与毕赤酵母分泌型表达载体ppicz a连接，构建ppicz a - ifn重组质粒，并用常规cacl _ 2法转化大肠杆菌jm109进行扩增。 |
| 7. | In 1987 , the notion of a splicing system was introduced by tom head in [ 3 ] as a mathematical model of restriction enzyme digestion and subsequence religation in the recombination of dna molecules
1987年， tomhead发表了一篇论文，引入拼接系统( splicingsystem )的概念作为限制酶与dna (脱氧核糖核酸)作用、进行dna重组的数学模型。 |
| 8. | Restriction enzyme digestion analysis and pcr analysis showed that these two plant expression vectors constructed were correct . 49caixin , one cultivar of the brassica campestris l . ssp . chinensis , was transformed by vacuum infiltration method
通过真空渗入和花序浸渍法，将上述双抗和单抗基因分别导入白菜不结球类型? ? 49菜心中，获得了7棵抗性株。 |
| 9. | 3 . the amplified dna fragment was then ligated into the hindlll and ecori sites of pmg36e . the ligation mixture was transformed into jm109 for the initial cloning . the recombinant plasmid pmg36e / nisa isolated from jm109 transformants were analyzed by restriction enzyme digestion
将酶切并纯化后的pcr扩增产物与大片段连接并转化e . colijm109 ，在含红霉素的lb平板上筛选到含有重组质粒的转化子。 |
| 10. | The gene fragments si and s2 were successfully amplified by pcr from plasmid pecob6 and were also successfully cloned into plasmid pbks ( + ) . and the recombinant plasmids were selected through identification with pcr , enzyme digestion methods , and detection of their sequences
成功从质粒pecob6特异性扩增了s1和s2的基因片段，分别将二者克降到质粒pbks ( + ) ，经过pcr 、双酶切攀定、序列测定等检测。 |
